ABSTRACT
Torque Teno Virus (TTV) of the genus Anelloviridae , is an unenveloped, circular DNA virus with 23 genotypes that has been shown to be a potential surrogate biomarker for "Net State of Immunosuppression" in organ transplantation. Additionally, TTV levels have been shown to correlate with acute rejection (AR) and chronic lung allograft dysfunction (CLAD) as well as response to CoVid-19 vaccine in lung transplantation. Donor-derived cell-free DNA (dd-cfDNA) is a validated biomarker known to detect AR, CLAD and infection in lung transplant (LT) patients. We hypothesize that plasma TTV levels may complement dd-cfDNA in detecting different immunologic events after LT. We prospectively collected plasma samples from LT recipients from a single center LT program. dd-cfDNA was analyzed and clinical-pathologic events were carefully adjudicated and curated in all lung transplant recipients to define AR, CLAD, infection and stable samples. Herein, we analyze these plasma samples by Next Generation Sequencing (NGS) with quantification for TTV. Analyses will examine time-dependent performance characteristics of both dd-cfDNA fraction and quantified TTV levels for the endpoints AR, CLAD and infection with stable samples used as the comparison group. [ABSTRACT FROM AUTHOR] Copyright of Journal of Heart & Lung Transplantation is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)